MiSeq System Denature and Dilute Libraries Guide (15039740 ) Author: Illumina Subject: Instructions for denaturing and diluting libraries before sequencing on the MiSeq system. Created Date: 2/20/2019 5:43:03 PM, 2/22/2019 · MiSeq System Denature and Dilute Libraries Guide Instructions for preparing PhiX and denaturing and diluting libraries for sequencing on the MiSeq.
12/17/2017 · MiSeq System Denature and Dilute Libraries Guide (15039740) Author: Illumina Subject: Instructions for denaturing and diluting libraries before sequencing on the MiSeq system. Created Date: 12/8/2017 3:26:11 PM, MiniSeq System Denature and Dilute Libraries Guide (1000000002697) Author: Illumina Subject: Instructions for denaturing and diluting libraries before sequencing on the MiniSeq system. Created Date: 12/16/2015 6:51:19 PM, MiSeq System Denature and Dilute Libraries Guide (document # 15039740) Provides instructions for denaturing and diluting prepared sample libraries before sequencing on the MiSeq, and preparing a PhiX control. This step applies to most library types. MiSeq Custom Primers Guide (document # 15041638) Provides instructions for preparing, NextSeq System Denature and Dilute Libraries Guide (15048776 ) Author: Illumina Subject: Instructions for denaturing and diluting libraries before sequencing on the NextSeq 500 and NextSeq 550 systems. Created Date: 12/17/2015 12:11:59 PM, 6/21/2017 · Pool, dilute , and denature samples* for an 8 pM** final concentration following the MiSeq Denature and Dilute Libraries Guide and the Index Pooling Guidelines. * The number of samples that can be pooled together will depend on the input amount, panel size, and number of reads required for each sample for the particular analysis being performed.
NextSeq System Denature and Dilute Libraries Guide (15048776 ) Author: Illumina Subject: Instructions for denaturing and diluting libraries before sequencing on the NextSeq 500 and NextSeq 550 systems. Created Date: 12/21/2018 4:56:33 PM, Denature and Dilute PhiX Control DenaturingandDilutingLibrariesfortheNextSeq500 7 DenatureandDilutePhiXControl UsethefollowingstepstodenatureanddiluteaPhiXcontrollibrary.
See MiSeq Applications and Methods for possible applications. After preparing libraries, denature and dilute libraries to a loading concentration of 6–20 pM. For instructions, see the MiSeq System Denature and Dilute Libraries Guide. Run Components. Use the compatible version of the MiSeq Control Software (MCS) for your flow cell.
